Immunoisolated Transplantation of Purified Langerhans Islet Cells in Testis Cortex of Male Rats for Treatment of Streptozotocin Induced Diabetes Mellitus     

Ali Farhangi  Dariush Norouzian  Mohammad Reza Mehrabi  Mohsen Chiani  Zahra Saffari  Maryam Farahnak  Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(4):406-417

The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food, volume of water, urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60 mg/kg dose of streptozotocin in 250–300 g (75–90 days) adult Wistar rats makes pancreas swell and causes degeneration in Langerhans islet β-cells and induces experimental diabetes mellitus in 2–4 days. For a microscopic study of degeneration of Langerhans islet β-cells of diabetic rats, biopsy from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. In this process, after collagenase digestion of pancreas, islets were isolated, dissociated and identified by dithizone method and then with enzymatic procedure by DNase and trypsin, the islet cells changed into single cells and β-cells were identified by immune fluorescence method and then assayed by flow-cytometer. Donor tissue in each step of work was prepared from 38 adult male Wistar rats weighted 250–300 g (75–90 days). Transplantation was performed in rats after 2–4 weeks of diabetes induction. In this study, the levels of insulin, C-peptide and glucose in diabetic rats reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells. Transplantation was performed under the cortex of testis as immunoisolated place for islet cells transplantation.  相似文献   

Knowledge transfer in alliances: the moderating role of the alliance type     

Hamid Mazloomi?Khamseh  Dominique?JollyEmail author 《知识管理研究与实践》2014,12(4):409-420

We analyse how the impact of determinants of inter-firm knowledge transfers in alliances can be moderated by the proximity of the resources pooled by partners. Six factors are examined: relative absorptive capacity (RAC), inter-partner trust, cultural distance between allies, knowledge ambiguity, knowledge protection, and the partners’ learning intent. We distinguish alliances mobilizing similar resources by partners (called ‘endogamies’ as allies build on their similarities) from alliances pooling different resources (called ‘exogamies’ as allies build on their differences). We propose that: (a) the impact of RAC on knowledge transfers is reinforced in exogamies, but reduced in endogamies; (b) the knowledge ambiguity and cultural distance effect is less in endogamies than in exogamies; (c) the trust and knowledge protection impact is stronger in exogamies than in endogamies; (d) the learning intent impact on knowledge transfers is similar in endogamies and exogamies.  相似文献   

Measurement and control of pressure driven flows in microfluidic devices using an optofluidic flow sensor     

Mohammad Sadegh Cheri  Hamidreza Shahraki  Jalal Sadeghi  Mohammadreza Salehi Moghaddam  Hamid Latifi 《Biomicrofluidics》2014,8(5)

Measurement and control of pressure-driven flow (PDF) has a great potential to enhance the performance of chemical and biological experiments in Lab on a Chip technology. In this paper, we present an optofluidic flow sensor for real-time measurement and control of PDF. The optofluidic flow sensor consists of an on-chip micro Venturi and two optical Fabry-Pérot (FP) interferometers. Flow rate was measured from the fringe shift of FP interferometers resulted from movement fluid in the on-chip micro Venturi. The experimental results show that the optofluidic flow sensor has a minimum detectable flow change of 5 nl/min that is suitable for real time monitoring and control of fluids in many chemical and biological experiments. A Finite Element Method is used to solve the three dimensional (3D) Navier–Stokes and continuity equations to validate the experimental results.  相似文献   

Teleconferencing: How to be a successful host     

Reza Azarmsa 《TechTrends》1987,32(4):19-23

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Beyond myths of Muslim education: a case study of two Iranian ‘supplementary’ schools in London     

Reza Gholami 《牛津教育评论》2017,43(5):566-579

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Symmetry properties of tetraammine platinum（Ⅱ） with C2v and C4v point groups     

Moghani GA  Ashrafi AR  Hamadanian M 《Journal of Zhejiang University. Science. B》2005,6(3):222-226

Let G be a weighted graph with adjacency matrix A=[aij]. An Euclidean graph associated with a molecule is defined by a weighted graph with adjacency matrix D=[d/ij], where for i≠j, dij is the Euclidean distance between the nuclei i and j. In this matrix dij can be taken as zero if all the nuclei are equivalent. Otherwise, one may introduce different weights for different nuclei.Balasubramanian (1995) computed the Euclidean graphs and their automorphism groups for benzene, eclipsed and staggered forms of ethane and eclipsed and staggered forms of ferroeene. This paper describes a simple method, by means of which it is possible to calculate the automorphism group of weighted graphs. We apply this method to compute the symmetry of tetraammine platinum(Ⅱ) with C2v and C4v point groups.  相似文献   

Effect of flushing on the detachment of biofilms attached to the walls of metal pipes in water distribution systems     

Jing-qing Liu  Zhi-feng Luo  Ke Liu  Yi-fu Zhang  Hong-xi Peng  Bao-lan Hu  Hong-xing Ren  Xiao-yan Zhou  Shang-de Qiu  Xiao-fang He  Ping Ye  Hamid Bastani  Li-ping Lou 《浙江大学学报(A卷英文版)》2017,18(4):313-328

Biofilm detachment caused by flushing can result in secondary contamination in drinking water distribution systems (DWDSs). To evaluate the impact of flushing on biofilm detachment, actual water supply pipes including ductile cast iron pipes (DCIPs), gray cast iron pipes (GCIPs), and stainless steel compound pipes (SSCPs) were used in this study. Real-time quantitative polymerase chain reaction and 454 pyrosequencing were used to quantify bacteria and analyse microbial community composition, respectively. The results showed that the pipe material greatly influences the resistance of a biofilm to flushing. Biofilms attached to DCIPs were able to resist quite strong flushing, while those attached to GCIPs and SSCPs were sensitive to flushing. Both flush-resistant and flush-sensitive bacteria were present in all the biofilms, but their frequency differed among the different metal pipes. Thus, the resistance to flushing of bacteria is related not only to the nature of the bacteria, but also to the pipe material. Although flushing can remove some of the biofilm and may be a good way to clean the DWDS, the shear stress needed to remove the biofilm differs among different pipe types. The results of this study provide technical support for the management and operation of DWDS.  相似文献   

Antagonistic interaction between <Emphasis Type="Italic">Trichoderma asperellum</Emphasis> and <Emphasis Type="Italic">Phytophthora capsici</Emphasis> in vitro     

Heng Jiang  Liang Zhang  Jing-ze Zhang  Mohammad Reza Ojaghian  Kevin D. Hyde 《Journal of Zhejiang University. Science. B》2016,17(4):271-281

Phytophthora capsici is a phytopathogen that causes a destructive pepper blight that is extremely difficult to control. Using a fungicide application against the disease is costly and relatively ineffective and there is also a huge environmental concern about the use of such chemicals. The genus Trichoderma has been known to have a potential biocontrol issue. In this paper we investigate the mechanism for causing the infection of T. asperellum against P. capsici. Trichoderma sp. (isolate CGMCC 6422) was developed to have a strong antagonistic action against hyphae of P. capsici through screening tests. The strain was identified as T. asperellum through using a combination of morphological characteristics and molecular data. T. asperellum was able to collapse the mycelium of the colonies of the pathogen through dual culture tests by breaking down the pathogenic hyphae into fragments. The scanning electron microscope showed that the hyphae of T. asperellum surrounded and penetrated the pathogens hyphae, resulting in hyphal collapse. The results show that seven days after inoculation, the hyphae of the pathogen were completely degraded in a dual culture. T. asperellum was also able to enter the P. capsici oospores through using oogonia and then developed hyphae and produced conidia, leading to the disintegration of the oogonia and oospores. Seven days after inoculation, an average 10.8% of the oospores were infected, but at this stage, the structures of oospores were still intact. Subsequently, the number of infected oospores increased and the oospores started to collapse. Forty-two days after inoculation, almost all the oospores were infected, with 9.3% of the structures of the oospores being intact and 90.7% of the oospores having collapsed.  相似文献   

THE EPISTEMIC VALUE OF CURIOSITY   总被引：1，自引：0，他引：1  

Frederick F. Schmitt  Reza Lahroodi 《Educational theory》2008,58(2):125-148

A bstract .  In this essay, Frederick Schmitt and Reza Lahroodi explore the value of curiosity for inquiry and knowledge. They defend an appetitive account of curiosity, viewing curiosity as a motivationally original desire to know that arises from having one's attention drawn to the object and that in turn sustains one's attention to it. Distinguishing curiosity from wonder, the authors explore several sources of the epistemic value of curiosity. First, curiosity is tenacious: curiosity whether a proposition is true leads to curiosity about related issues, thereby deepening our knowledge. Second, it is to some extent biased in favor of topics in which we already have a practical or epistemic interest. Third, and most important, curiosity is largely independent of our interests: it fixes our attention on objects in which we have no antecedent interest, thereby broadening our knowledge. Schmitt and Lahroodi elucidate the value of curiosity by outlining its role in levels of development — an approach indebted to John Dewey's explanation of the value of curiosity. Finally, they raise some questions about the implications of their account for educational practice.  相似文献   

Variations in cadmium and nitrate co-accumulation among water spinach genotypes and implications for screening safe genotypes for human consumption     

Lin Tang  Wei-jun Luo  Zhen-li He  Hanumanth Kumar Gurajala  Yasir Hamid  Kiran Yasmin Khan  Xiao-e Yang 《Journal of Zhejiang University. Science. B》2018,19(2):147-158

Vegetables are important constituents of the human diet. Heavy metals and nitrate are among the major contaminants of vegetables. Consumption of vegetables and fruits with accumulated heavy metals and nitrate has the potential to damage different body organs leading to unwanted effects. Breeding vegetables with low heavy metal and nitrate contaminants is a cost-effective approach. We investigated 38 water spinach genotypes for low Cd and nitrate co-accumulation. Four genotypes, i.e. JXDY, GZQL, XGDB, and B888, were found to have low co-accumulation of Cd (<0.71 mg/kg dry weight) and nitrate (<3100 mg/kg fresh weight) in the edible parts when grown in soils with moderate contamination of both Cd (1.10 mg/kg) and nitrate (235.2 mg/kg). These genotypes should be appropriate with minimized risk to humans who consume them. The Cd levels in the edible parts of water spinach were positively correlated with the concentration of Pb or Zn, but Cd, Pb, or Zn was negatively correlated with P concentration. These results indicate that these three heavy metals may be absorbed into the plant in similar proportions or in combination, minimizing the influx to aerial parts. Increasing P fertilizer application rates appears to prevent heavy metal and nitrate translocation to shoot tissues and the edible parts of water spinach on co-contaminated soils.  相似文献